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KMID : 0368420060490060448
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Journal of Plant Biology
2006 Volume.49 No. 6 p.448 ~ p.448
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Determination of self-locompatibility genotypes of Korean apple cultivars based on S-RNase PCR
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Kim Hoy-Taek
Hattori Gen
Hirata Yutaka
Kim Dae-Ill
Hwang Jeong-Hwan
Shin Yong-Uk
Nou Ill-Sup
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Abstract
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To prevent self-fertilization, apple has a gametophytic self-incompatibility mechanism, part of a widespread intraspecific system, that is controlled by a multi-allelic locus. This attribute has been exploited in breeding programs for new cultivars. Likewise, many apple orchards depend on artificial pollination. Therefore, molecular analysis and early identification of the self-incompatibility (S) genotype could greatly improve breeding schemes and pollen donors selection. Here, we PCR-amplified the S-RNase PCR fragments from a total of 14 cultivars and parents, using new primers (ASPF3+ASPR3) common to 23 S-alleles in apple. The S-genotypes were determined for the following: ‘Hongro’ (S1S3), ‘Gamhong’ (S1S9), ‘Saenara’ (S1S3), ‘Chukwang’ (S3S9), ‘Hwahong’ (S3S9), ‘Seokwang’ (S3S3), ‘Hwarang’ (S1S9), ‘Sunhong’ (S3S9), ‘S.E.B.’ (S1S19), ‘S.G.D.’ (S2S3), and ‘Mollie’s Delicious’ (S3S7). We also confirmed the characteristics of the S-genotypes for eight Korean apple cultivars by PCR-Southern blot analysis, using seven S-RNases as probes.
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KEYWORD
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apple, gametophytic self-incompatibility, PCR, S-genotype, S-RNase
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